To develop, validate, and apply a single nucleotide polymorphism (SNP) microarray–based method for simultaneous preimplantation genetic diagnosis (PGD) of unbalanced inheritance of rearranged chromosomes and 24-chromosome aneuploidy screening.Prospective clinical research study.Academic reproductive medicine center.Eighteen couples carrying a balanced reciprocal or Robertsonian chromosomal rearrangement.PGD on blastocyst trophectoderm biopsy specimens.Aneuploidy, implantation, pregnancy, and delivery rates after SNP microarray–based aneuploidy and translocation screening.Single nucleotide polymorphism microarray was capable of detecting translocation-associated imbalances as small as 9.0 megabases. In the 12 transfers performed, sustained implantation occurred for 9 (45%) of 20 balanced-normal and euploid embryos replaced. The clinical pregnancy rate in patients receiving a transfer was 75% with six singleton deliveries and three ongoing singleton pregnancies thus far. Significantly fewer embryos were eligible for transfer with the incorporation of simultaneous 24-chromosome aneuploidy screening. Arrested embryos were also significantly more likely to possess unbalanced chromosomes when compared with developmentally competent blastocysts.This SNP microarray–based method provides the first opportunity to improve outcomes through comprehensive identification of euploid embryos from translocation carrier couples.