To examine whether more paired corticospinal–motoneuronal stimulation (PCMS) is more effective at inducing spinal level plasticity.To produce facilitation, corticospinal volleys evoked by motor cortical transcranial magnetic stimulation (TMS) were timed to arrive at corticospinal–motoneuronal synapses prior to antidromic potentials evoked in motoneurones by electrical brachial plexus stimulation. Paired stimuli were delivered repeatedly. 50-pair conditioning (50-PCMS) was compared to 100 pairs in single block (100-PCMSsingle) and spaced (2 blocks of 50, 15-min break; 100-PCMSspaced) patterns, and to 50 single, unpaired TMS (50-TMS). Biceps responses to cervicomedullary stimulation (cervicomedullary motor evoked potentials, CMEPs) and TMS (motor evoked potentials, MEPs) were measured before and for 1h after conditioning (recorded each 5min).After 100-PCMS, average CMEP areas were increased by 46±55% (mean±SD; n=10; 100-PCMSsingle) and 71±99% (100-PCMSspaced). 50-PCMS produced a non-significant 6±40% increase. After 100-PCMSsingle and 100-PCMSspaced, CMEPs were larger than those after 50-TMS from 0 to 60min (p<0.05). 100-PCMSsingle and 100-PCMSspaced produced more reliable changes than 50-PCMS. Overall, MEPs were larger at 35-60min; however there were no differences between conditioning protocols.More PCMS produces more reliable enhancement of corticospinal transmission.This technique has therapeutic potential to improve muscle control in patients with reduced descending drive.