The Y-STRs DYS481, DYS570, DYS576, and DYS643 were investigated in a West Saxonian population sample, which have been previously typed with the well known minimal or extended haplotype of Y-STRs. Observed allele frequencies are reported along with the allele nomenclature based on sequencing. Gene diversities as well as the haplotype diversity of the four markers – DYS481, DYS570, DYS576 and DYS643 – were calculated and compared with published data of other population samples. The discrimination capacities of the markers show a high potential for forensic purposes.