Bioselective adsorption was evaluated as a possible technology for food processing to enhance safety usingBacillus cereusin milk as a model system. Cataphote™ Microbeads class −400 were derivatized by attaching 3-aminopropyl groups (2.7 nm 2 /molecule) onto the surface of the bead. Carbohydrates on the Fc region of monoclonal antibody 183 againstB. cereusT spores were oxidized with potassium meta-periodate to allow for an oriented antibody immobilization (270 nm 2 /molecule). The adsorption matrix was characterized for its ability to bindB. cereusspores in comparison to a control matrix containing immobilized bovine serum albumin. When 2.5 × 10 6 spores in skim milk were added to 1 mL of each matrix, the IgG-matrix was capable of removing 96% of that amount, 70% of which were bound with high affinity and were only eluted with 0.1 mol/L acetic acid. In contrast, the control matrix removed 90% of the spores added but only 7% were retained after washing the matrix. In addition, the IgG-matrix showed an excellent regeneration ability; the binding level did not decrease significantly after 28 trials with buffer or milk. Calculations determined that the bioadsorbant was capable of removing 8 × 10 6 spores/m 2 . Thus, bioselective adsorption has promise as a technology to enhance safety of liquid foods or to improve analytical methodology.