A reinvestigation of Alstroemeria revoluta afforded, in addition to tuliposides A and D, a new tuliposide. The structure of the new tuliposide was determined by spectral methods to be 1-[4-(4-hydroxy-2-methylenebutanoate)-2-methylenebutanoate]-6-(4-hydroxy-2-m ethylenebutanoate)-β-d-glucopyranose. The investigation of four other Alstroemeria species revealed only the presence of tuliposides A and D.