The effects of the application of an acoustically assisted treatment on the vitamins (C, B1, B2, B3, and B5), the antioxidant activity (DPPH, FRAP), the polyphenol and flavonoid contents, the organic acid contents (citric and malic) and drying kinetics of pineapple (Ananas comosus var. Perola) have been studied. Treatments were carried out using two different soaking media: distilled water and pineapple juice at 30°C during 10, 20 and 30min without and with acoustic assistance (23.2W/L). After treatment, samples were dried at 60°C and 0.5m/s during 8h. The quality parameters were determined in untreated, treated, and treated-dried samples. The acoustic assistance promoted an increment of vitamins B1, B2, B3 and B5, total flavonoid and malic acid contents, and a reduction of vitamin C, total polyphenol content, antioxidant activity and citric acid content in treated samples. However, in all treated-dried samples the final content of those quality parameters was higher than the observed in the untreated dried sample.