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Fluorescein isothiocyanate-labeled chitosan was synthesized as a macromolecular fluorophore to investigate the molecular mechanism of interactions between chitosan and mucin by the fluorescence polarization method. The percent change in polarization upon chitosan–mucin association was measured as a function of the molecular weight of chitosan, and the pH and ionic strength of the medium. Dextran sulfate...
Previous work has shown strong interactions between pig gastric mucins and a highly deacetylated chitosan. Recently, mucins purified from different areas of the porcine stomach have been shown to differ in terms of their oligosaccharide substitution and net charge. How this regional variation may affect the properties of these mucins is of great interest in terms of the specificity of mucoadhesion...
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