Journal of Pharmaceutical and Biomedical Analysis

Items from 1 to 20 out of 42 results

article

Comparison of the quantification of acetaminophen in plasma, cerebrospinal fluid and dried blood spots using high-performance liquid chromatography–tandem mass spectrometry

Rachel R. Taylor, Keith L. Hoffman, Björn Schniedewind, Claudia Clavijo, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 1-9

Acetaminophen (paracetamol, N-(4-hydroxyphenyl) acetamide) is one of the most commonly prescribed drugs for the management of pain in children. Quantification of acetaminophen in pre-term and term neonates and small children requires the availability of highly sensitive assays in small volume blood samples.We developed and validated an LC–MS/MS assay for the quantification of acetaminophen in human...

article

1H NMR-based metabolomics studies of urine reveal differences between type 1 diabetic patients with high and low HbAc1 values

Stanislaw Deja, Ewa Barg, Piotr Młynarz, Aleksander Basiak, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 43-48

The aim of this study was to investigate relation between level of HbAc1 and concentration of metabolites in urine of T1D patients. To test this hypothesis the ¹ H NMR (proton nuclear magnetic resonance) target analysis of crucial urine metabolites combined with chemometric approach were applied. Urine samples were collected from 30 children and teenagers aged 4–19 with T1D and 12 healthy...

article

Ternary system of dihydroartemisinin with hydroxypropyl-β-cyclodextrin and lecithin: Simultaneous enhancement of drug solubility and stability in aqueous solutions

Dan Wang, Haiyan Li, Jingkai Gu, Tao Guo, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 141-148

The purpose of this study was to simultaneously improve the solubility and stability of dihydroartemisinin (DHA) in aqueous solutions by a ternary cyclodextrin system comprised of DHA, hydroxypropyl-β-cyclodextrin (HP-β-CD) and a third auxiliary substance. Solubility and phase solubility studies were carried out to evaluate the solubilizing efficiency of HP-β-CD in association with various auxiliary...

article

The profiling and identification of the absorbed constituents and metabolites of Paeoniae Radix Rubra decoction in rat plasma and urine by the HPLC–DAD–ESI-IT-TOF-MSn technique: A novel strategy for the systematic screening and identification of absorbed constituents and metabolites from traditional Chinese medicines

Jing Liang, Feng Xu, Ya-Zhou Zhang, Shuai Huang, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 108-121

Paeoniae Radix Rubra (PRR, the dried roots of Paeonia lactiflora) is a commonly used traditional Chinese medicine (TCM). A clear understanding of the absorption and metabolism of TCMs is very important in their rational clinical use and pharmacological research. To find more of the absorbed constituents and metabolites of TCMs, a novel strategy was proposed. This strategy was characterized by the...

article

Antigen digestion on the target plate of MALDI-TOF MS after isolation using an immunoaffinity membrane

Youji Shimazaki, Yuri Nishimura, Masaki Saito

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 293-298

A combination of methods is required to achieve separation of intact proteins and subsequently perform structure analysis to examine their unstable or external structures. The aim of this study was to develop a method of structure analysis in intact proteins after purification. Transferrin from human plasma was trapped by membrane-immobilized anti-transferrin antibody, which was produced by non-denaturing...

article

Development and validation of a fast chromatographic method for screening and quantification of legal and illegal skin whitening agents

B. Desmedt, V. Rogiers, P. Courselle, J.O. De Beer, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 82-88

During the last years, the EU market is flooded by illegal cosmetics via the Internet and a so-called “black market”. Among these, skin-bleaching products represent an important group. They contain, according to the current European cosmetic legislation (Directive 76/768/EEC), a number of illegal active substances including hydroquinone, tretinoin and corticosteroids. These may provoke as well local...

article

Development and application of a novel, dual-mode gradient, stability-indicating HPLC-DAD method for the simultaneous determination and purity assessment of mebeverine hydrochloride, diloxanide furoate and their corresponding major degradation products in combination with some gastrointestinal drugs in the form of oral doses

Mokhtar Mabrouk, Hamed El-Fatatry, Ismail Hewala, Ehab Emam

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 249-259

A simple, precise, rapid, stability-indicating reversed phase high performance liquid chromatographic method with photodiode array detection was developed and validated for the determination of mebeverine hydrochloride in combination with sulpiride or with diloxanide furoate and metronidazole in the presence of their corresponding degradation products. Optimum separation was achieved in less than...

article

On-line near infrared spectroscopy as a Process Analytical Technology (PAT) tool to control an industrial seeded API crystallization

C. Schaefer, C. Lecomte, D. Clicq, A. Merschaert, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 194-201

The final step of an active pharmaceutical ingredient (API) manufacturing synthesis process consists of a crystallization during which the API and residual solvent contents have to be quantified precisely in order to reach a predefined seeding point. A feasibility study was conducted to demonstrate the suitability of on-line NIR spectroscopy to control this step in line with new version of the European...

article

High resolution reversed phase analysis of recombinant monoclonal antibodies by ultra-high pressure liquid chromatography column coupling

Szabolcs Fekete, Michael W. Dong, Taylor Zhang, Davy Guillarme

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 273-278

Monoclonal antibodies (mAbs) represent one of the fastest growing areas of new drug development. However, their analytical characterization is complex and generally requires an array of orthogonal analytical techniques. Reversed phase liquid chromatography is a valuable strategy due to its high resolving power and straightforward compatibility to mass spectrometry. The present study demonstrates that...

article

Biorelevant solubility of poorly soluble drugs: Rivaroxaban, furosemide, papaverine and niflumic acid

Krisztina Takács-Novák, Vera Szőke, Gergely Völgyi, Péter Horváth, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 279-285

In this work the biorelevant solubility of four drugs representing different acid–base property, wide range of lipohilicity and low aqueous solubility was studied. The equilibrium solubility of rivaroxaban (non-ionizable), furosemide (acid), papaverine (base) and niflumic acid (ampholyte) was determined in simulated gastric fluid (SGF pH 1.2), in simulated intestinal fluid fasted state (FaSSIF pH...

article

Hyaluronidases and their inhibitors in the serum of colorectal carcinoma patients

C. Kolliopoulos, D. Bounias, H. Bouga, D. Kyriakopoulou, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 299-304

Colorectal cancer is the third most commonly diagnosed type of cancer. Hyaluronan is involved in this malignancy. Moreover, hyaluronidases – its degrading enzymes – display controversial roles regarding their involvement in tumor development. HYAL-1 is the major tumor derived hyaluronidase. The aim of the study was the determination and evaluation of hyaluronidase levels in serum of colorectal cancer...

article

A UFLC–MS/MS method coupled with one-step protein precipitation for determination of docetaxel in rat plasma: Comparative pharmacokinetic study of modified nanostructured lipid carrier

Xu Zhao, Kaishun Bi, Xiaofan Wang, Xiao Xue, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 202-208

A rapid, simple and sensitive ultra fast liquid chromatography–tandem mass spectrometry (UFLC–MS/MS) method coupled with one-step protein precipitation procedure has been developed and validated for the pharmacokinetic study of docetaxel in rat plasma to investigate the influence of polyethylene glycol (PEG) molecular weights (chain length) using in the modified formulations. Separation was achieved...

article

Detection and quantification of cimicoxib, a novel COX-2 inhibitor, in canine plasma by HPLC with spectrofluorimetric detection: Development and validation of a new methodology

Mario Giorgi, Tae-Won Kim, Alessandro Saba, Mohammad-Reza Rouini, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 28-33

Cimicoxib (CX) is a selective COX-2 inhibitor recently launched on the veterinary market. No analytical method to detect CX in biological samples has been published to date.The chromatographic separation was performed with a Kinetex C ₁₈ analytical column (100mm×4.6mm, 2.6μm particle size) at 25°C. The mobile phase consisted of acetonitrile:buffer (10mM AcONH ₄ , pH 4.5) (35:65, v/v)...

article

Pharmacokinetics and tissue distribution of a bioactive sesquiterpenoid from Polygonum jucundum following oral and intravenous administrations to rats

Fei Zhang, Xinshi Gong, Baiming Xiao, Chaofeng Zhang, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 135-140

Polygonum jucundum is a traditional Chinese medicine used for the treatment of diseases. The major bioactive compound in the ethanol extract of P. jucundum is 2α-hydroxyl-3β-angeloylcinnamolide (HAC), a drimane-type sesquiterpenoid, which possesses anti-inflammatory activities. The purpose of this study was to investigate in vivo pharmacokinetics and tissue distribution of HAC after oral and intravenous...

article

A novel methodology for analysis of enantiomers through determination of their critical micelle concentrations using spectrophotometric method

Ghodratollah Absalan, Yousef Alipour

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 96-100

A novel methodology for analysis of enantiomer composition of chiral compounds based on measuring of their critical micelle concentration by using spectrophotometric technique is introduced. The proposed method does not require any chiral selector and the corresponding process is simple, fast, reproducible, and inexpensive. The procedure was successfully applied for analysis of naproxen. The critical...

article

Stability of sample solution as a crucial point during HPLC determination of chemical purity of temozolomide drug substance

Łukasz Jedynak, Maria Puchalska, Marta Zezula, Marta Łaszcz, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 19-27

An HPLC method for determination of related substances in temozolomide drug substance was developed. Particular attention was paid to the stability studies due to the fact that temozolomide is unstable in a solution and quickly decomposes to its main degradation product 5-amino-4-imidazolecarboxamide (AIC). A mixture of diluted acetic acid and acetonitrile (4:1, v/v) as a diluent guaranteed lowering...

article

A multivariate-based wavenumber selection method for classifying medicines into authentic or counterfeit classes

Michel J. Anzanello, Rafael S. Ortiz, Renata P. Limbergerb, Paulo Mayorga

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 209-214

Attenuated total reflectance (ATR), a sampling technique by Fourier transform infrared (FTIR) spectroscopy, has been adopted as an analytical tool for detecting fraudulent medicines. The spectrum generated by FTIR-ATR typically relies on hundreds of equally spaced wavenumbers which may reduce the performance of techniques tailored to classify samples into classes, i.e., authentic or fraudulent. This...

article

Investigation of monoclonal antibody fragmentation artifacts in non-reducing SDS-PAGE

Zhiqing C. Zhu, Yingchen Chen, Michael S. Ackerman, Bei Wang, more

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 89-95

Fragmentation of monoclonal antibodies has been routinely observed in non-reducing SDS-PAGE, mainly due to disulfide-bond scrambling catalyzed by free sulfhydryl groups, resulting in a method induced artifact. To minimize this artifact, alkylating agents like iodoacetamide (IAM) and N-ethylmaleimide (NEM) were commonly included in SDS sample buffer to block free sulfhydryls. However, the selection...

article

Comparative metabolism study of β-lapachone in mouse, rat, dog, monkey, and human liver microsomes using liquid chromatography–tandem mass spectrometry

Sangkyu Lee, In Sook Kim, Tae Hwan Kwak, Hye Hyun Yoo

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 286-292

β-Lapachone (3,4-dihydro-2,2-dimethyl-2H-naphthol[1,2-b]pyran-5,6-dione) is a natural compound extracted from the bark of the lapacho tree (Tabebuia avellanedae) and is undergoing phase II clinical trials as an antitumor drug candidate. The present study characterized in vitro metabolites of β-lapachone in mouse, rat, dog, monkey and human liver microsomes. β-Lapachone (10μM) was incubated with mouse,...

article

Crystal structure and dehydration behavior of E6070, a novel IKKβ protein kinase inhibitor for the treatment of rheumatoid arthritis

Ikuo Kushida

Journal of Pharmaceutical and Biomedical Analysis > 2013 > 83 > Complete > 221-227

This study is focused on solving the crystal structure of E6070, a novel IKKβ protein kinase inhibitor, and characterizing its solid state. E6070 exists as a stable crystalline trihydrate (hydrate I) that undergoes two dehydration events on heating. Neither crystal form nor water content changed under the relative humidity range from 11% to 93% at 25°C. Crystal transformation to hydrate II occurred...

Number:
Complete
Volume:
83
Year:
2013

Publication date

Set your own date range

Keywords

PHARMACOKINETICS (5)
HPLC (4)
LC–MS/MS (4)
HUMAN PLASMA (3)
METABOLOMICS (3)
STABILITY (3)
DIHYDROARTEMISININ (2)
ION MOBILITY SPECTROMETRY (2)
LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY (2)
MASS SPECTROMETRY (2)
QUANTIFICATION (2)
VALIDATION (2)
(EPI)CATECHIN (1)
10-HYDROXYPRAZIQUANTEL (1)
1H NMR SPECTROSCOPY (1)
2Α-HYDROXYL-3Β-ANGELOYLCINNAMOLIDE (HAC) (1)
5-AMINOSALICYLIC ACID (1)
ACETAMINOPHEN (1)
ACTIVATION ENERGY (1)
ACTIVE PHARMACEUTICAL INGREDIENT (API) CRYSTALLIZATION (1)
ADENOMA (1)
ALKYLATION AGENTS (1)
AMLODIPINE (1)
ARTESUNATE (1)
ATORVASTATIN (1)
BIOANALYSIS (1)
BIOEQUIVALENCE (1)
BIORELEVANT SOLUBILITY (1)
BIOSIMILAR (1)
BLEACHING AGENTS (1)
BLOOD (1)
BMS-927711 (1)
C3-KETOSTEROIDS (1)
CERVI CORNU (1)
CFSE (1)
CGRP ANTAGONIST (1)
CHEMICAL CONSISTENCY (1)
CHEMICAL FINGERPRINT (1)
CIMICOXIB (1)
CLEANING AGENTS (1)
CLEANING VALIDATION (1)
COATING DEFECTS (1)
COLISTIN (1)
COMPARATIVE METABOLISM (1)
CORRELATION ANALYSIS (1)
CORTICOSTEROIDS (1)
COUNTERFEIT MEDICINES (1)
CRITICAL MICELLE CONCENTRATION (1)
DA-CHENG-QI-TANG (1)
DAUNORUBICIN (1)
DEGRADATION (1)
DEHYDRATION (1)
DIACETYL MIDECAMYCIN (1)
DIETARY SUPPLEMENT (1)
DIRECT DESORPTION (1)
DISSOLUTION (1)
DOCETAXEL (1)
DOGS (1)
DRIED BLOOD SPOTS (1)
DRUG SUBSTANCE (1)
DUAL-MODE GRADIENT (1)
EFFECTIVE FORMS (1)
ELAPHURI DAVIDIANI CORNU (1)
ELECTRONIC TONGUE (1)
ENANTIOMER COMPOSITION (1)
ENDOPROTEINASE LYS-C (1)
FASSIF (1)
FESSIF (1)
FLUORESCENCE (1)
FOOD EFFECT (1)
FRAGMENT-BAND ARTIFACT (1)
FTIR-ATR (1)
FUNCTIONAL COATING (1)
GALLIC ACID (1)
GLYCATED HEMOGLOBIN (HBA1C) (1)
GLYCOFORM PROFILE (1)
HIGH RESOLUTION SEPARATIONS (1)
HIGH TEMPERATURE (1)
HILIC-UHPLC–TQ-MS/MS (1)
HUMAN CSF (1)
HYAL-1 (1)
HYALURONAN METABOLISM (1)
HYALURONIDASE INHIBITORS (1)
HYDRATE (1)
HYDROQUINONE (1)
HYDROXYPROPYL-Β-CYCLODEXTRIN (1)
ICH GUIDELINE Q2 (1)
IGG4 (1)
ILLEGAL COSMETICS (1)
ILLICIT ERECTILE DYSFUNCTION PRODUCTS (1)
INSTABILITY (1)
ION-PAIRING AGENTS (1)
KNN (1)
LC/ESI-MS (1)
LC–MS (1)
LIVER MICROSOMES (1)
MABS (1)
MALDI-TOF MS (1)
MALIGNANCY (1)
MATHEMATICAL CORRECTION (1)
more

INFONA - science communication portal

Journal of Pharmaceutical and Biomedical Analysis

Comparison of the quantification of acetaminophen in plasma, cerebrospinal fluid and dried blood spots using high-performance liquid chromatography–tandem mass spectrometry

1H NMR-based metabolomics studies of urine reveal differences between type 1 diabetic patients with high and low HbAc1 values

Ternary system of dihydroartemisinin with hydroxypropyl-β-cyclodextrin and lecithin: Simultaneous enhancement of drug solubility and stability in aqueous solutions

Antigen digestion on the target plate of MALDI-TOF MS after isolation using an immunoaffinity membrane

Development and validation of a fast chromatographic method for screening and quantification of legal and illegal skin whitening agents

On-line near infrared spectroscopy as a Process Analytical Technology (PAT) tool to control an industrial seeded API crystallization

High resolution reversed phase analysis of recombinant monoclonal antibodies by ultra-high pressure liquid chromatography column coupling

Biorelevant solubility of poorly soluble drugs: Rivaroxaban, furosemide, papaverine and niflumic acid

Hyaluronidases and their inhibitors in the serum of colorectal carcinoma patients

A UFLC–MS/MS method coupled with one-step protein precipitation for determination of docetaxel in rat plasma: Comparative pharmacokinetic study of modified nanostructured lipid carrier

Detection and quantification of cimicoxib, a novel COX-2 inhibitor, in canine plasma by HPLC with spectrofluorimetric detection: Development and validation of a new methodology

Pharmacokinetics and tissue distribution of a bioactive sesquiterpenoid from Polygonum jucundum following oral and intravenous administrations to rats

A novel methodology for analysis of enantiomers through determination of their critical micelle concentrations using spectrophotometric method

Stability of sample solution as a crucial point during HPLC determination of chemical purity of temozolomide drug substance

A multivariate-based wavenumber selection method for classifying medicines into authentic or counterfeit classes

Investigation of monoclonal antibody fragmentation artifacts in non-reducing SDS-PAGE

Comparative metabolism study of β-lapachone in mouse, rat, dog, monkey, and human liver microsomes using liquid chromatography–tandem mass spectrometry

Crystal structure and dehydration behavior of E6070, a novel IKKβ protein kinase inhibitor for the treatment of rheumatoid arthritis

Filter options

Publication date

Keywords

INFONA - science communication portal

Journal of Pharmaceutical and Biomedical Analysis

Add recipient

Sending message cancelled

Are you sure you want to cancel sending this message?

Send message

Filter options

Publication date

Date range setting

Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.

Keywords

Reporting an error / abuse

Sending the report failed

Accessibility options