Kα to Lα and Kβ to Lβ intensity ratios for elements Ba, La, Ce, Nd, Sm, Tb, and Dy have been measured at the excitation energy of 59.54 keV γ-rays from 2 4 1 Am radioactive source of strength 100 mCi. Intensity ratios were determined by measuring K and L X-rays emitted from standard target of a given element. Theoretical values of the Kα to Lα and Kβ to Lβ intensity ratios were calculated using theoretically tabulated values of shell/subshell photoionization cross-sections, fluorescence yields, Coster-Kronig transition probabilities and radiative decay rates for η K L i <>0 and η K L i =0. Experimental results were compared with the theoretical values.