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The gene of the non-structure protein 2 (NS2) was cloned by PCR from the genome of Bombyx mori densovirus Zhenjiang strain (BmDNV-Z), inserted into prokaryotic expression vector pET28a to construct recombinant plasmid pET28a-NS2 and then expressed in bacteria Escherichia coli BL21 (DE3). The expressed recombinant protein was identified by SDS-PAGE and Western blot analysis. Then, the recombinant protein...
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