Thermoinhibition in Tagetes minuta achenes is tightly and rapidly regulated with regard to its imposition and release, with both processes occurring within 2–3h. Germination at high temperatures is almost exclusively regulated by the embryo, while the pericarp appears to play only a minor role. Thermoinhibition in T. minuta could not be alleviated by any single plant growth regulator application, but a combination of treatments that both reduced ABA levels and increased ethylene levels were able to restore germination at supraoptimal temperatures. This suggests a role for both ethylene and ABA in the imposition of thermoinhibition in this species.