Silk has attractive fiber features including its pearl-like gloss, a light velvety touch, which makes it a fashionable and comfortable material for apparel. These beautiful characteristics are derived from the skillful spinning of the silkworm. The larvae, Bombyx mori controls the molecular orientation of the silk fiber using several complicated spinning techniques. In order to clarify the mechanisms of silk fiber formation, the interaction of behavior, morphology, and chemistry in the process were investigated. Liquid silk fibroin was obtained from the silk glands of full-grown larvae of bombyx mori one day before spinning or cocooning. For examination of the gel-sol transition spinning and high speed spinning, samples were prepared from fibroin obtained from the anterior, middle, and posterior part of the middle division. For examination of the liquid crystal and gel structure of fibroin, the specimens were prepared from the anterior division of the silk gland. Specimens were also prepared from the spinneret region in order to investigate the self-exerted spinning, zone elongation and porous spinning, drying spinning and crimp spinning methods. Silkworms control the molecular orientation accurately by using a multiple combination of sophisticated spinning techniques, such as gel spinning, liquid crystal spinning, high-speed spinning, self exerted (traction) spinning, zone elongation, porous spinning, ion spinning, dry spinning, crimp spinning, and low energy spinning.