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Monte Carlo simulations were used to reveal a subtle, hidden contribution to van't Hoff enthalpy estimates from small heat capacity changes. In simulated van't Hoff plots of ln K versus 1/T, small heat capacity changes (-200 ≤ ΔC p ≤ + 200 cal mol -1 K -1 ), for which curvature could not be discerned within the noise of the data, were found to systematically bias...
Calorimetric measurements of absolute heat capacity have been performed for hydrated 11 S-globulin (O <C H2O < 25%) and for lysozyme in a concentrated solution, both in the native and denatured states. The denaturation process is observed in hydrated and completely anhydrous proteins; it is accompanied by the appearance of heat capacity increment (Δ D ...
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