A seemingly routine paternity case which involved the testing of a mother, child and man led to inconclusive results as an exclusion involving only a single repeat was found at one of the 14 loci tested. This led to the testing of further loci. Only the single exclusion was found after profiling a total of 21 loci, with the addition of a further single locus providing a second exclusion. Even with both mutation events incorporated into the calculation a paternity index (PI) of 4957 was calculated, still a significant figure. However, when a second likelihood ratio was calculated assessing the likelihood of the results if the biological father of the child was the tested man or the tested man's brother, then the results were not significant, only 0.15. This analysis led to the profiling of the tested man's brother who matched at all 19 loci that were profiled and was concluded to be the biological father.