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Cellobiose dehydrogenase (CDH) is an emerging enzyme that is being investigated for applications in many fields including biosensors, biofuels, bioremediation, and textile processing and recently for several biomedical applications. This review summarizes the recent advances in the application of CDH in the biomedical field, particularly as a novel antimicrobial and antibiofilm agent, as biosesensor,...
A gene encoding cellobiose dehydrogenase (VvCDH) from Volvariella volvacea was successfully expressed in Pichia pastoris with codon optimization using its native signal sequence. VvCDH had optimum pH and temperature at 5.5 and 60°C respectively and showed a broad range of pH stability between 5 and 8. Kinetic analysis showed that the best substrate is cellobiose and that the Km for celloolgosaccharides...
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