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Purified endoglucanase (apparently to homogeneity) from Arachniotus citrinus was immobilized in polyacrylamide gel with 53.4% yield of immobilization. Immobilization improved the pH optimum from 4.9 (free) to 5.6 (immobilized) and temperature optimum by 10 o C. The V max of immobilized endoglucanase at 70 and 90 o C were 1.4- and 3.0-fold improved over its free counterpart...
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