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An Escherichia coli acid phosphatase gene with phytase activity was cloned into pBBRT plasmid and transformed into Pseudomonas putida. A complex medium was developed for phytase production from this recombinant strain. After cultivation at 30 o C for 48h crude phytase was isolated and characterized. The optimum temperature and pH for activity were 55 o C and pH 4.0, respectively. Enzyme...
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