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Chryseobacterium gleum exhibited complete dissolution of whole chicken-feathers (10gl −1 , pH 8) after 72h at 30°C through synthesis of keratinolytic protease when inoculated at 1% (v/v). This enzyme was purified to 67-fold with yield of 2.25% having a specific activity of 1670Umg −1 and ∼36kDa Mw. MALDI-TOF MS of this keratinase showed some similarity with the keratinase peptides...
Alaska pollock skin as aquatic by-product was usually discarded in food processing. Iron deficiency has been a nutritional problem for a long period. In this study, Alaska pollock skin was used to generate iron-chelating peptides after being treated by commercial enzymes. Sequential chromatography, including immobilized metal affinity chromatography (IMAC) and reversed phase high performance liquid...
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