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Subtilisin QK (QK), which is highly homologous to nattokinase (NK), has comparatively high thrombolytic activity on both heated plasma and fibrin plate. The aim of this study was to produce QK in Pichia pastoris (P. pastoris) and establish improved techniques for its fermentation, purification and lyophilisation. QK was expressed as a secretory protein by P. pastoris; the thrombolytic activity of...
In development of yeast cell factories, the adequate stress tolerance of the yeast strains is the universal requirement for industrial fermentation. Primary stress factor is the end product toxicity which has to be accumulated to obtain economically feasible performance in industrial processes. Second stress factor is the concentration and composition of substrate used for ethanol production and the...
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