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An aqueous two-phase system consisting of methanol and phosphate was used to extract 1,3-propanediol from glycerol-based fermentation broth. Methanol not only extracted 1,3-propanediol effectively from the broth through forming aqueous two-phase system with phosphate, but also allowed the recovery of phosphate by dilution crystallization through adjusting the pH. At 35% (v/v) methanol, saturated concentration...
Sugaring-out is a novel phase separation method which was found firstly in the separation of acetonitrile. Nowadays sugaring-out extraction was tried in the recovery of metal ions, biomolecules, drugs and proteins, while its application in separation of hydrophilic primary metabolites from fermentation broths was unknown. In this work sugaring-out extraction of 2,3-butanediol was explored. The effects...
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