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P MAL1 , the sigma 70-like sequences possessing promoter of the maltase gene of Hansenula polymorpha, was evaluated for its application in heterologous protein production in Escherichia coli. Levansucrases Lsc1, Lsc2 and Lsc3 of Pseudomonas syringae pv. tomato DC3000 were expressed from P MAL1 in E. coli as biotechnologically relevant model proteins. Production of soluble levansucrases...
An intermittent feeding strategy in fed-batch cultivation has been widely applied to control the accumulation of overflow toxic metabolites and oxygen limitation. However, successive depletions of carbon source by carbon-limited feeding might induce considerable stress to host cells, such as nutrient starvation, which may evoke the enhanced degradation of secreted recombinant proteins by proteolysis...
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