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A thermo-alkaline pectate lyase (BliPelA) gene from an alkaliphilic Bacillus licheniformis strain was cloned and overexpressed in Escherichia coli. Mature BliPelA exhibited maximum activity at pH 11 and 70°C, and demonstrated cleavage capability on a broad range of substrates such as polygalacturonic acid, pectins, and methylated pectins. The highest specific activity, of 320Umg−1, was towards polygalacturonic...
Acetoin is a natural flavor with potential applications in food, pharmaceutical, and chemical industries. However, the titer of acetoin in fermentation production of this compound is still relatively low, which hinders its large-scale industrial production. In this work, fermentative production of acetoin by Bacillus licheniformis WX-02 was optimized through metabolic engineering and fermentation...
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