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The Na + -translocating NADH:quinone oxidoreductase (Na + -NQR) from the human pathogen Vibrio cholerae is a respiratory flavo-FeS complex composed of the six subunits NqrA–F. The Na + -NQR was produced as His 6 -tagged protein by homologous expression in V. cholerae. The isolated complex contained near-stoichiometric amounts of non-covalently bound FAD (0.78 mol/mol...
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