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To better understand the molecular mechanisms that underlie the exaggerated bradykinin (BK)-stimulated release of Ins(1,4,5)P 3 in fibroblasts from Alzheimer patients, the role of G-proteins, protein kinase C (PKC) and cyclic AMP in BK-induced Ins(1,4,5)P 3 formation was determined. A role for G-proteins in the coupling of the BK receptor to intracellular signals was indicated by...
We were to examine the effect of bradykinin (BK) in the regulation of glutamate transporter and its related signaling molecules in a human retinal pigment epithelial (ARPE) cells, which are important cells to support retina.d-[2,3- 3 H]-aspartate uptake, western immunoblotting, reverse transcription polymerase chain reaction, [ 3 H]-arachidonic acid release, and siRNA transfection...
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