Varus thrust is a gait deviation and a known risk factor for knee osteoarthritis. Prevalence of varus thrust and normative values of stance-phase knee deviation across the lifespan are unknown. The aim of the study was to report prevalence of varus thrust and normative values for hip-knee-ankle (HKA) angle deviation across the lifespan, and to explore associations between HKA angle deviation and selected clinical factors.This is a cross-sectional study of 572 participants from the 1000 Norms Project. People aged 3–101 years who self-reported as being healthy were eligible to participate. Video recordings (2D) of frontal plane gait were analysed for presence of varus or valgus thrust and quantification of HKA angle deviation (difference between HKA angles at initial contact and mid-stance). Correlation and multiple regression analyses explored the relationship between HKA angle and age, sex, body mass index (BMI), alignment, knee and hip strength, Knee Injury and Osteoarthritis Outcomes Scores (KOOS), foot posture index, gait parameters, and hypermobility.Thirty-one percent (31%) of the cohort had varus thrust. This was most prevalent among adults>60 years (42%) and children<10 years (41%), and was also common in adolescents (25%) and adults aged 20–59 (23%). Mean HKA angle deviation for the entire cohort was 1.2° (95% CI: 1.07–1.36), and 2.1° (95% CI: 1.84–2.36) among people with clinical varus thrust. BMI, stride width, KOOS-Sports for adolescents, and height for adults were independently associated with HKA angle deviation.Varus thrust is prevalent across the lifespan. Normative values established here can be readily used by clinicians and researchers in monitoring this deviation.