Drosophila is a very favorable animal model for the studies of neuroscience. However, it remains a great challenge to employ electrophysiological approaches in Drosophila to study the neuronal assembly dynamics in vivo, partially due to the small size of the Drosophila brain. Small and sensitive microelectrodes for multi-unit recordings are greatly desired.We fabricated micro-scale stereotrodes for electrical recordings in Drosophila melanogaster. The stereotrodes were modified with iridium oxide (IrO 2 ) under a highly controllable deposition procedure to improve their electrochemical properties. Electrical recordings were carried out using the IrO 2 stereotrodes to detect spontaneous action potentials and LFPs in vivo.The IrO 2 electrodes exhibited significantly higher capacitance and lower impedance at 1kHz. Electrical recording with the IrO 2 stereotrodes in vivo demonstrated an average signal-to-noise ratio (SNR) of 7.3 and a significantly improved LFP sensitivity. 5 types of different neurons recorded were clearly separated. Electrophysiological responses to visual and odor stimulation were also detected, respectively.The most widely used electrodes for electrical recording in Drosophila are glass microelectrode and sharpened tungsten microelectrode, which are typically used for single-unit recordings. Although tetrode technology has been used to record multi-neuronal activities from Drosophila, the fabricated IrO 2 stereotrodes possess smaller geometry size but exhibited comparable recording signal-to noise ration and better sorting quality.The IrO 2 stereotrodes are capable to meet the requirements of multi-unit recording and spike sorting, which will be a useful tool for the electrophysiology-based researches especially in Drosophila and other small animals.