Introduction: CD2 is expressed on murine B cells, probably as a result of a chromosomal translocation event during speciation. There are no activating antibodies to mouse CD2, but when lymphocytes are activated non specifically, only T cells upregulate their expression of CD2. Activating antibodies have been well characterised for human CD2.Materials and Methods: We investigated the expression and function of CD2 on B cells using mice transgenic for human CD2 under the control of a modified version of the autologous promoter/enhancer that included the immunoglobulin enhancer. This construction directs expression of human CD2 to the B cell compartment as well as to the T cell compartment.Results: Here, we confirm that activating pairs of anti CD2 monoclonal antibodies are mitogenic for mouse T cells transgenic for human CD2. In contrast, mouse B cells that express similar amounts of human CD2 are not stimulated to proliferate by equivalent doses of these antibodies. We were also unable to show any functional consequence for these B cells as a result of CD2 ligation.Conclusion: These data powerfully suggest that CD2 does not act to transduce a signal in B cells, they do not impact on the question of whether the molecule plays a role in cellular adhesion.