Journal of Chromatography B

Items from 1 to 13 out of 13 results

article

Characterization of the Clostridium difficile volatile metabolome using comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry

Christiaan A. Rees, Aimee Shen, Jane E. Hill

Journal of Chromatography B > 2016 > 1039 > C > 8-16

Clostridium difficile is a bacterial pathogen capable of causing life-threatening infections of the gastrointestinal tract characterized by severe diarrhea. Exposure to certain classes of antibiotics, advanced age, and prolonged hospitalizations are known risk factors for infection by this organism. Anecdotally, healthcare providers have reported that they can smell C. difficile infections in their...

article

Corrigendum to “Simultaneous measurement of etravirine, maraviroc and raltegravir in pigtail macaque plasma, vaginal secretions and vaginal tissue using a LC–MS/MS assay” [J. Chromatogr. B 1025 (2016) 110–118]

Anna K. Blakney, Yonghou Jiang, Dale Whittington, Kim A. Woodrow

Journal of Chromatography B > 2016 > 1039 > C > 88

article

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Journal of Chromatography B > 2016 > 1039 > C > i

article

HPLC-based metabolomics to identify cytotoxic compounds from Plectranthus amboinicus (Lour.) Spreng against human breast cancer MCF-7Cells

Wahid Yulianto, Nuri Andarwulan, Puspo Edi Giriwono, Joko Pamungkas

Journal of Chromatography B > 2016 > 1039 > C > 28-34

The objective of this study was to identify the active compounds in Plectranthus amboinicus (Lour.) Spreng which play a role to inhibit viability of breast cancer MCF-7 cells using HPLC-based metabolomics approach. Five fractions of the plant extract were observed including ethanol, hexane, chloroform, ethyl acetate and water fraction. There were 45 HPLC chromatograms resulted from 5 fractions with...

article

Use of response surface methodology for partitioning, one-step purification of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031)

Amaal M. Alhelli, Mohd Yazid Abdul Manap, Abdulkarim Sabo Mohammed, Hamed Mirhosseini, more

Journal of Chromatography B > 2016 > 1039 > C > 66-73

This report shows the partitioning and purification of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031) by solid-state fermentation (SSF). In the present analysis, some of the important parameters such as PEG concentration, PEG molecular mass, salt concentration and buffer concentration were optimised through the response surface methodology (RSM). The optimum aqueous two-phase...

article

Determination of trans-resveratrol and its metabolites in rat serum using liquid chromatography with high-resolution time of flight mass spectrometry

Jana Rousova, Kari Kusler, Duminda Liyanage, Mary Leadbetter, more

Journal of Chromatography B > 2016 > 1039 > C > 35-43

In this study we developed a sensitive method using high performance liquid chromatography (HPLC) coupled to electrospray ionization (ESI) with high resolution time of flight (TOF) mass spectrometry (MS) for the determination of naturally occurring antioxidant trans-resveratrol (3,5,4′-trihydroxy-trans-stilbene, RES). This method enabled an investigation of a relationship between tumor growth in rats...

article

Fully automated two-step assay for detection of metallothionein through magnetic isolation using functionalized γ-Fe2O3 particles

Miguel Angel Merlos Rodrigo, Ludmila Krejcova, Jiri Kudr, Natalia Cernei, more

Journal of Chromatography B > 2016 > 1039 > C > 17-27

Metallothioneins (MTs) are involved in heavy metal detoxification in a wide range of living organisms. Currently, it is well known that MTs play substantial role in many pathophysiological processes, including carcinogenesis, and they can serve as diagnostic biomarkers. In order to increase the applicability of MT in cancer diagnostics, an easy-to-use and rapid method for its detection is required...

article

Validated quantification method for five ephedrines in dietary supplements using LC–MS/MS: Application to 503 cases

Jianli Zhang, Yinong Zhang, Yan Wang

Journal of Chromatography B > 2016 > 1039 > C > 1-7

Ephedrines in dietary supplements can arise from herbs or illegal adulteration so a liquid chromatography tandem mass spectrometry (LC–MS/MS) method was developed for separation and quantification of ephedrine, pseudoephedrine, norephedrine, norepseudoephedrine, and methyl-ephedrine, some of which are isomer pairs of pseudo-structures. This method includes liquid–liquid extraction of ephedrines from...

article

Saponins from Panax bipinnatifidus Seem.: New strategy of extraction, isolation, and evaluation of tyrosinase inhibitory activity based on mathematical calculations

Yuchi Zhang, Chong Shi, Chunming Liu, Min Yu, more

Journal of Chromatography B > 2016 > 1039 > C > 79-87

A hyphenated accelerated solvent extraction (ASE) technique coupled with centrifugal partition chromatography (CPC), ultra high performance liquid chromatography (UPLC), and mass spectrometry (MS) was established. The CPC fractions were prepared using the hyphenated technique. Subsequently, tyrosinase inhibitory activities of the CPC fractions were experimentally evaluated, and the activities of individual...

article

Purification and characterization of CRISP-3 from human seminal plasma and its real-time binding kinetics with PSP94

Jenifer H. Anklesaria, Rushika R. Pandya, Bhakti R. Pathak, Smita D. Mahale

Journal of Chromatography B > 2016 > 1039 > C > 59-65

Cysteine-rich secretory proteins (CRISPs) have been postulated to have a role in male reproduction and prostate pathophysiology. Of the mammalian CRISPs, CRISP-3 levels in particular have been shown to be upregulated in prostate cancer. Efforts have been made to obtain highly pure CRISP-3 for gaining structure-function information of this protein. However, well characterized and highly pure protein...

article

A highly sensitive assay of human plasma xanthine oxidoreductase activity using stable isotope-labeled xanthine and LC/TQMS

Takayo Murase, Mai Nampei, Mitsuru Oka, Atsushi Miyachi, more

Journal of Chromatography B > 2016 > 1039 > C > 51-58

Associations between elevated plasma xanthine oxidoreductase (XOR) activity and various pathologies have been widely reported. However, it has been difficult to accurately measure human plasma XOR activity because the XOR activity of humans is lower than that of animals such as mouse. We developed a highly sensitive assay for XOR activity utilizing a combination of [¹³C₂,¹⁵N₂] xanthine and liquid...

article

Unbound fraction of fluconazole and linezolid in human plasma as determined by ultrafiltration: Impact of membrane type

Alexander Kratzer, Frieder Kees, Christoph Dorn

Journal of Chromatography B > 2016 > 1039 > C > 74-78

Ultrafiltration is a rapid and convenient method to determine the free concentrations of drugs in plasma. Several ultrafiltration devices based on Eppendorf cups are commercially available, but are not validated for such use by the manufacturer. Plasma pH, temperature and relative centrifugal force as well as membrane type can influence the results. In the present work, we developed an ultrafiltration...

article

Mathematical modeling of the whole expanded bed adsorption process to recover and purify chitosanases from the unclarified fermentation broth of Paenibacillus ehimensis

Carlos Eduardo de Araújo Padilha, Paulo Victor Fortunato Dantas, Francisco Canindé de sousa, Domingos Fabiano de Santana Souza, more

Journal of Chromatography B > 2016 > 1039 > C > 44-50

In this study, a general rate model was applied to the entire process of expanded bed adsorption chromatography (EBAC) for the chitosanases purification protocol from unclarified fermentation broth produced by Paenibacillus ehimensis using the anionic adsorbent Streamline^® DEAE. For the experiments performed using the expanded bed, a homemade column (2.6cm×30.0cm) was specially designed. The proposed...

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1039
Year:
2016

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Keywords

ACCELERATED SOLVENT EXTRACTION (1)
ANTIINFECTIVES (1)
AQUEOUS TWO PHASE SYSTEMS (1)
AUTOMATION (1)
BACTERIAL VOLATILES (1)
BINDING PROTEIN (1)
BIOACTIVE COMPOUNDS (1)
BIOMARKERS (1)
CENTRIFUGAL PARTITION CHROMATOGRAPHY (1)
CHITOSANASES (1)
CLOSTRIDIUM DIFFICILE (1)
CLOSTRIDIUM DIFFICILE-ASSOCIATED DIARRHEA (1)
CRISP-3 (1)
CYTOTOXICITY (1)
DIETARY SUPPLEMENTS (1)
DOPING-CONTROL (1)
ELECTROSPRAY IONIZATION (1)
EPHEDRINE ALKALOIDS (1)
EXPANDED BED ADSORPTION (1)
EXPERIMENTAL DESIGN (1)
FERMENTATION BROTH (1)
GC×GC-TOFMS (1)
HEADSPACE VOLATILES (1)
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (1)
HIGH RESOLUTION TIME OF FLIGHT MASS SPECTROMETRY (1)
HPLC (1)
HUMAN PLASMA (1)
HUMAN SEMINAL PLASMA (1)
LIPASE (1)
LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC-MS/MS) (1)
MAGNETIC ISOLATION (1)
MALDI-TOF MS (1)
MASS SPECTROMETRY (1)
MATHEMATICAL MODELING (1)
MATHEMATICS (1)
METABOLOMICS (1)
METALLOTHIONEIN (1)
PANAX BIPINNATIFIDUS (1)
PATIENTS (1)
PENICILLIUM CANDIDUM (1)
PLECTRANTHUS AMBOINICUS (LOUR.) SPRENG (1)
POLYETHERSULFONE (1)
POLYPHENOLS (1)
PROSTATE (1)
PROTEIN BINDING (1)
PSP94 (1)
PURIFICATION (1)
RAT SERUM (1)
REGENERATED CELLULOSE (1)
RESVERATROL (1)
RSM (1)
SOLID STATE FERMENTATION (1)
STABLE ISOTOPE LABELED SUBSTRATE (1)
TYROSINASE INHIBITOR (1)
VOLATILE METABOLOMICS (1)
XANTHINE OXIDOREDUCTASE (1)
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Journal of Chromatography B

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