Diagnostic laparoscopic is the most appropriate method to approach children with bilateral non-palpable testes as imaging studies are not considered to be effective any more. However, some authors advocate endocrinological evaluation beforehand since laparoscopy is an invasive method. The serum basal antimüllerian hormone(AMH) and human chorionic gonadotropin stimulation test are the most commonly used two endocrinological methods. We performed bilateral orchiectomy to simulate anorchia in new born rats and compared inhibin B and AMH levels of them with those of the bilateral cryptorchid and normal rats.Totally 108 Sprague-Dawley rats(3 groups of 36 each) were included in this study. Bilateral testis were placed and fixed to the intraabdominal region after birth in the first group. Bilateral orchiectomy was performed after birth in the second group. Third group was the control group. All rats were sacrified in 1, 3, 7, 14, 21 and 35th day and serum samples were collected with intracardiac aspiration.Serum inhibin B and AMH levels were measured.Mean serum inhibin B and AMH levels were 113,6pg/ml and 3,38 ng/ml, respectively in the first group and 129,1pg/ml and 3,4 ng/ml, respectively in the third group. On the 3rd day of orchiectomy, both inhibin B and AMH decreased to unmeasurable levels (mean inhibin B 0,887pg/ml and AMH 0,64pg/ml) in the second group. There was no significant difference between those two hormone levels in terms of sensitivity and specifity (p<0.005).Testicular tissue existence could be determined with the measurement of serum basal inhibin B and AMH levels.