In previous work, large high quality crystals of tetragonal lysozyme were grown using constant supersaturation control (CSC) growth algorithms. Working toward the goal of inducing increased temperature sensitivity in protein crystallization, these results have been extended to low ionic strength systems using salts such as NaSCN and NaNO 3 in lieu of NaCl. Using the combination of calorimetrically-determined growth rate parameters and constant supersaturation temperature control algorithms; large, well formed, single crystals have been grown in NaSCN and NaNO 3 buffer systems. The improvement in quality of these lysozyme crystals upon employing temperature controlled growth is striking. When compared to crystals grown using more traditional methods these crystals are quite large, and show few, or no, obvious defects. Preliminary crystal quality assessment via X-ray diffraction studies are encouraging. A single monoclinic CSC grown crystal had an average signal to noise ratio of 8.4 at a resolution of 1.62Å. In addition, this crystal showed excellent resistance to radiation damage and continued to diffract well after more then 45h of continuous beam exposure.