The present work describes radiation-induced effects on surface microorganisms on some stored seeds widely used in India. Co-60 gamma source at 25 o C emitting gamma ray at 1173 and 1332keV energy was used for irradiating the rice (Oryza sativa. Cv-2233 and Oryza sativa. Cv-Shankar) and pulse (Cicer arietinum. Cv-local) having >10% moisture content. Dose of gamma irradiation up to 6kGy (0.12kGy/h) was applied for exposing the seeds. Significant depletion of the fungal population on seeds was noted with irradiation at 1-2kGy, while complete inhibition of the contaminating fungi was observed above 4kGy. Though the germinating potential of the treated grains did not alter significantly, loss in membrane permeability of the exposed seeds was significant.