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The effect of succinate on the production of cloned glucoamylase by a recombinant yeast system was investigated. Saccharomyces cerevisiae was transformed with the 2 μ-based plasmid YEpSUCSTA containing the SUC2 promoter, the STA signal sequence, and the STA structural gene. Specific glucoamylase expression was 89% higher in a semi-synthetic medium containing succinate than in medium without succinate...
Fermentation characteristics of Saccharomyces cerevisiae ATCC 9763 immobilised in Ca-alginate gel beads have been investigated in a stirred batch system at 2, 4, 8, 10% (w/v) initial glucose concentrations. The experimental results were tested using eleven different kinetic models relating biomass and ethanol production and glucose utilisation in different forms. The models proposed by Monod and...
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