The ion-acoustic instability in a dusty negative ion plasma is investigated, focusing on the parameter regime in which the negative ion density is much larger than the electron density. The dynamics of the massive dust grains are neglected, but collisions of electrons and ions with dust grains in addition to other collisional processes are taken into account. The presence of a population of charged dust can change the frequency of the fast wave, lead to additional damping due to ion–dust collisions, and change the conditions for wave growth. Applications to dusty negative ion plasmas in the laboratory and in space are discussed.