To evaluate the repetition suppression (RS) in resting and active motor evoked potential (MEPs) and in corticospinal silent period (cSP) using navigated transcranial magnetic stimulation (nTMS). MEPs can be though to represent the activity of the excitatory system, whereas SPs represent the inhibitory effect of the GABAergic interneurons.Seven healthy right-handed subjects (4 females, age: 24–35years) were studied. nTMS was focused on the left cortical representation of first dorsal interosseous muscle by applying stimulus trials including 4 stimuli at 1s intervals. Total of 30 trials were given 20s apart. Resting MEPs were measured with a stimulation intensity of 120% of the resting motor threshold. Active MEPs and cSPs were measured during voluntary muscle contraction at 120% of the silent period threshold (SPT) with 20 trials.RS was evidenced in the resting MEPs (F=44.11, p<0.001), whereas no RS was observed with the active MEPs (p=0.193). Instead of RS, the cSPs significantly lengthened during the stimulation trials (F=19.97, p<0.001).While the resting MEPs demonstrate RS, active MEPs stay intact and SPs lengthen indicating potential intracortical inhibition as the mechanism of RS.RS appears specific to resting MEPs and may occur due to increasing intracortical inhibition.