In this study, kinetic analyses were conducted for two Brocadia-dominated enrichment cultures, granular and flocculent, retrieved from lab-scale anaerobic ammonium oxidation (anammox) reactors. Substrate K S ranged from 0.35 to 0.69mMN and V Smax ranged from 0.67 to 0.88mmolNg −1 VSSh −1 . The model respected the experimentally measured stoichiometry of N-compounds, serving as an independent validation. Growth kinetics of the flocculent sludge was also studied, which indicates a μ max of 0.0984d −1 and a Y X/S of 0.105mol C-biomass mol −1 NH4+. The flocculent enrichment culture was used to determine the stoichiometric equation. The kinetic parameters of the Brocadia spp. cultures measured here can be used for optimizing applications of the anammox process.