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Barley beta -amylase exhibits two distinct band patterns after isoelectric focusing (IEF), termed Sd1 and Sd2. A comparison of the deduced amino acid sequences revealed five amino acid differences between the two types ofbeta -amylase. To investigate whether the two band patterns are due to these amino acid substitutions, four Sd2 mutants (Sd2-R115C, Sd2-D165E, Sd2-L347S and Sd2-V430A) were constructed...
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