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The only unequivocal method for diagnosing sheep and goats infection due to Brucella melitensis is bacterial isolation on appropriate culture media (i.e. the Farrell and modified Thayer-Martin selective media). PCR based on different molecular markers (16S rRNA, bscp31, IS 6501/711) has been shown to be a valuable method for detecting Brucella DNA and provides a promising option. For large-scale surveillance/eradication...
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