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In order to achieve successful fertilization, the essential steps include maturation, species-specific binding and fusion of gametes followed by activation of the ovum. Implicit in this are a variety of molecular events, the most important among them being the receptor-ligand interaction. A protocol is described to isolate highly enriched fractions of goat sperm plasma and the outer acrosomal membranes...
A major obstacle in successful cooled semen storage is the reactive oxygen species generated in the media by the sperm cells. Recently, many reports document the antioxidant, anti-carcinogenic and anti-mutagenic properties of flavonoids when used in vitro or in vivo. The objective of this study was to determine whether supplementing diluents with the flavonoids, silibinin or catechin, would aid in...
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