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The objective of this study was to compare the effectiveness of different activation treatments of in vitro matured oocytes and their developmental potency in potassium simplex optimization medium. Ovaries were collected from the local abattoir and transported within 4h to the laboratory in warm saline (37°C) containing 100IU penicillin-G and 100μg streptomycin sulphate per ml. A total of 1004 cumulus...
We investigated the effect of KL and IGF-I, alone or in combination, on the in vitro survival, growth and antrum formation of sheep preantral follicles, and subsequent oocyte developmental competence. Preantral follicles ∼200μm were isolated and cultured for 18 days in basic medium (control) added or not by KL, IGF-I or both. Every six days follicular survival, growth and antrum formation was evaluated...
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