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The aim of this study was to test the effect of insulin–transferrin–selenium (ITS), l-ascorbic acid (AA) and different hormone concentrations at IVM on blastocyst development, MPF activity and ATP content in lamb oocytes. In this study we used three maturation media: conventional IVM medium (CM), growth medium (GM: CM+ITS+AA and low level of hormones) and modified CM (MM: CM+ITS+AA and conventional...
In this study, Small Tail Han sheep embryos were produced in the breeding and non-breeding seasons by in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). Oocyte recovery and maturation rate, embryo development and blastocyst cell number were evaluated in a series of four experiments using either a 2 treatment (breeding vs. non-breeding season)×5 replicates or a 2 seasons×2 embryo...
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