The purpose of this study was to investigate differences in fat metabolism between visceral fat obese (VF-Ob) and abdominal subcutaneous obese (SF-Ob) men during “high-intensity endurance exercise”. Fourteen obese (body mass index >25kg/m 2 ) men were classified into two groups according to visceral fat area using computed tomography; i.e., VF-Ob (n=7; mean age, 52.0±2.5 year) and SF-Ob (n=7; mean age, 57.3±2.8 year) groups. Plasma fat concentration and fat oxidation were measured at rest and during 60-min high-intensity (70% of peak oxygen uptake) stationary cycling exercise. Plasma concentrations of free fatty acid and glycerol were significantly higher (P≤0.05) in VF-Ob men compared with SF-Ob men during endurance exercise. However, no significant difference was found in fat oxidation between VF-Ob and SF-Ob men (697±135 and 661±96kJ/h, respectively) during high-intensity endurance exercise. These results suggest that obesity phenotype affects plasma fat concentration even during high-intensity exercise. It is likely that plasma fat concentrations in visceral fat obese men during high-intensity endurance exercise are more increased compared with during moderate-intensity endurance exercise. Despite the difference in plasma fat concentration, total fat oxidation was similar in the two obese phenotypes.