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Transparent TiO2 nanoparticle films have been prepared by dropping TiO2/tetrahydrofuran dispersions on polystyrene (PS) culture dish followed by a dry process at 60°C for 2h. The resulting TiO2 film showed high visible light transmittance and the wettability was transformed from hydrophobic (water contact angle=91.2°) to superhydrophilic (water contact angle=0°) by using UV irradiation. NCTC Clone 929 cells were attached and well spread on the TiO2 film coated PS culture dish and can be detached rapidly from the surface after 20min UV illumination due to the photo-induced wettability conversion of TiO2 film. The present PS films modified by TiO2 nanoparticles would have potential application in light-induced cell harvest.