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To express the soluble recombinant hemangiopoietin protein in E. coli BL21(DE3). Using human fetal live cDNA as a template, a partial cDNA fragment of HAPO coding N-terminal region was subcloned into plasmids pTrc99, pQE60 and pET32c to construct different recombinant prokaryotic expression systems. After selecting, the soluble rhHAPO fusion protein was expressed stably in E. coli BL21 (DE3) by...
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