We investigated the emission of the secondary ions stimulated by single impacts of 136keV Au 400 4+ projectiles. The study was carried out on targets of glycine, phenylalanine, and C 60 . In addition, a target of C 60 was examined with 18keV C 60 + projectiles. The experiments were performed in the event-by-event bombardment/detection mode. The secondary ions were identified with linear time-of-flight mass spectrometer equipped with an 8-anode detector. The Au 400 4+ projectile induces abundant multi-ion emission, for instance the average number of detected ions (atomic, fragment, molecular and cluster ions) emitted per event from glycine target is 12.5. The glycine intact molecular ion (Gly − ) yield is 1.14. The bombardment of a C 60 target results in the efficient emission of multiple intact C 60 − (total yield is 0.15).