Laser Doppler flowmetry for microcirculation monitoring was performed in 10 healthy volunteers (mean age ± SD: 24.9 ± 3.3 years; 5 females, 5 males) prior to, during, and after stimulation using a noninvasive violet (405 nm) laser needle at the Dazhui (GV14) acupoint. The results of this controlled study (control point at the shoulder) showed significant (p < 0.05) increases in Flux (product of concentration and velocity of erythrocytes) at a distance of 3 cm from the acupoint. At the same time, no changes were found at the control point. Furthermore, 5 minutes after stimulation the microcirculation still flowed at a higher level than baseline values at the beginning of the investigation. In a single individual it was demonstrated that needle acupuncture enhanced this effect and placebo (deactivated laser) did not have a significant effect. The results suggest that violet laser acupuncture at the acupoint Dazhui can increase vascular effects on microcirculation.