Among the large amount of xenobiotics polluting the environment, polychlorinated phenols have been in the limelight in recent years.Studies dealing with chronic toxicity of some phenol congeners confirm their cancerogenic (hepatocellular tumors, leukemia) and immunosuppressive properties. Their elimination from the organism occurs via urine either in the free form or in the form of sulphate and glucuronide conjugates.The method is described for the isolation, derivatization, separation, and GC determination of chlorinated phenols in urine. After acid hydrolysis, solid phase extraction on Separcol SI C 1 8 was used. The recovery of the method range from 72.3 [plusmn] 9.9 to 109.9 [plusmn] 6.3%, the limit of determination varied from 0.0005 to 0.002 [mu ]g ml [ m i n u s ] 1 .The aim of this study was to define the exposure of occupationally and non-occupationally exposed population to chlorinated phenols. The levels of chlorinated phenols were several times higher in the group of occupationally exposed workers. The highest median levels in a group of 28 occupationally exposed workers were found for PCP, TeCP, and 2,4,5-TCP (0.074, 0.039, and 0.038 [mu ]g ml [ m i n u s ] 1 respectively). The median levels of chlorophenols in the control group ranged from ND (not detected) to 0.019 [mu ]g ml [ m i n u s ] 1 .