Background: Gαq is a member of the Gq family of G proteins, which by stimulating the phospholipase Cβ (PLCβ)-IP 3 -Ca + + mediated intracellular signaling systems controls some of the most fundamental cardiovascular cellular processes. The study of Gαq is complicated by the presence of a pseudogene in human DNA, with signficant homology to the mRNA encoding the α subunit of Gq protein. The presence of this pseudogene will cause problems when the analysis of the Gαq gene expression is based solely on RT-PCR. In this study, we report a simple method for avoiding unwanted amplification of the Gαq pseudogene and the use of human monocytes as a readily available source for examining Gαq. Methods: RT-PCR was carried out on RNA extracted from peripheral blood monocytes of 10 normal subjects using specific primers for Gαq. Results: When several subjects' Gαq was examined, the authentic Gαq mRNA amplification product levels, as a ratio to unpurified pseudogene containing amplification products, declined by up to approximately 70%. Conclusions: Given the importance of Gq protein in cardiovascular signal transduction, it is fundamental to provide a reliable assessment of Gαq gene expression. In addition to accurately assessing Gαq levels, the use of circulating human monocytes as a useful source of Gαq for investigating mechanisms involved in the regulation of vascular tone is shown.