We describe the cloning of a near full-length cDNA of 4258 nucleotides encodingfreac-9(HGMW-approved symbolFKHL17), a novel human forkhead gene. The 5′ untranslated region is unusual since it is very long, 2127 nucleotides, and contains 15 upstream AUG codons. Hybridization to a panel consisting of RNA derived from 50 different tissues showed thatfreac-9is transcribed exclusively in the kidney. The kidney-derived cell lines COS-7 and 293 are shown to expressfreac-9.A combination of fluorescencein situhybridization and somatic cell hybrids localizesfreac-9to the chromosomal region of 1p32–p34. The conceptual translation product predicts a protein of 372 amino acids with an N-terminal domain rich in acidic amino acids and with a high likelihood of forming an amphipatic helix, a DNA binding forkhead domain, and a C-terminal region that has a high probability of forming an amphipatic β-sheet. The amino acid sequence of the DNA binding forkhead motif of FREAC-9 is identical to that of another forkhead protein, FREAC-4, whereas 12 substitutions are present at the nucleotide level. There are no similarities in regions outside of the DNA binding domains of FREAC-9 and FREAC-4 and sincefreac-4maps to a different chromosome (5q12–q13) it is likely that an evolutionary selection has acted to maintain identical DNA binding domains between these two kidney expressed transcription factors.