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Expression levels of mRNA are commonly measured as a ratio of test to reference gene. The assumption is that reference genes such as β-actin or cyclophilin are unaffected by treatment and act as steady-state controls. TaqMan real-time RT-PCR was used to test these assumptions in a rat model of cerebral ischaemia (tMCAO). Following measurement of 24 genes, we show that reference genes in this animal...
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