Currents flowing through slow vacuolar SV channels of rape (Brassica napus) growing on media supplemented with Cd 2+ (400μM), and/or SeO 4 2− (2μM) were examined. The aim of the study was to investigate the role of Cd 2+ in modulation of SV channel activity and to determine whether Se reverses the effect of cadmium. Vacuoles were isolated using a quick surgical method to avoid application of any cell wall-degrading enzymes. Vacuoles of rape exhibited typical SV channel activity with slow activation at positive potentials and strong rectification into the vacuolar lumen. Single-channel conductance in cytoplasm-side-out tonoplast patches ranged between 68.8±1.9pS in the control, 80.1±2.5pS, in Cd 2+ -, 74.2±2.4pS in Cd 2+ /selenate-, and 80.1±1.8pS in selenate-pretreated plants. The lack of a clear tendency was likely an effect of equilibration of the pipette solution (without Cd 2+ /SeO 4 2− ) with that of the luminal side of the vesicles. In the vacuole-attached configuration, in which natural vacuolar solution was not exchanged, there was a significant reduction in single-channel conductance in the Cd 2+ - (40.3±2.8pS), Cd 2+ /selenate- (47.1±2.8pS) and selenate-pretreated (42.3±1.4pS) plants, compared to the control (60.2±1.7pS). The reduction in single-channel conductance only partially explained the significant decline in the densities of ion current flowing through the vacuolar membrane in the whole-vacuole configuration in the plants growing on Cd 2+ and Cd 2+ /selenate media. Thus, Cd 2+ accumulation in the vacuole reduced the number of active SV channels from 0.28±0.05μm −2 in the control to 0.021±0.005 and 0.039±0.004μm −2 in Cd 2+ and Cd 2+ /selenate-pretreated plants, respectively.